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Anaerobic Petri Dish Technique
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» kavita_raja - Re: Anaerobic Petri Dish Techniques In response to message posted by royermid:The method in common use employs a disc of filter paper having the same diameter as a petri dish.It is placed on the dorsum of the lid and a mixture of pyrogallol and sodium carbonate (1:3)in powder form is spread on it. The inoculated plate is inverted over the filter paper and sealed tight with molten wax.The dry pyrogallol mixture is activated by the moisture within the closed system,and complete anaerobiosis develops within about two hours.Better results,when kept in a candle jar or CO2 incubator.Ref :Textbook of Microbiology,BY R.Ananthanarayanan and C.K.Jayaram Panicker Orient Longman 5th edition 1999 -- posted by kavita_raja » bensabio - Re: Re: Anaerobic Petri Dish Techniques Thanks for this! I just arrived at this site for the first time and found a solution to a problem I was looking for had just been posted!!I was looking for an inexpensive way to provide an anaerobic environment as an alternative to the expensive GasPak chamber which we can't afford at this time. I know that Oxyrase makes a system (reducing enzyme and special plates) that is supposed to work. But i wondered if anyone has tried to use some kind of airtight container that is not as expensive as the commercial GasPak system. What about a plastic bag (ziplock?) of some kind? Are there any that are airtight? A quick trial in my kitchen, mixing baking soda and vinegar in a closed ziplock to generate CO2 ended up with a pretty flabby bag overnight. Any other ideas? Thanks again. -- posted by bensabio
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